The subject is a microcontroller chip I have used for such things in the past. It has two virtues. It is flat, and it has lots of fine detail. The chip is an ancient Intel 8748 -- an 8 bit controller that found extensive use in keyboards in times long past.
I am shooting with my Canon R5, which is a full frame mirrorless camera. I am using a Raynox 150 as a relay lens. I am using electronic flash (my Canon 580ex speedlight) for illumination.
The R5 sensor is 8192 by 5465 pixels, so if I want to extract a section 1200 pixels wide, I will sort of need to eyeball 1/8 or so of the sensor width and then crop. Doing that gives what I show next, where I pick a part of the image that shows the best focus.
Adobe lightroom makes it a total pain in the ass to do this, so this is not the exact 1200 pixels I might like, but it is verbatim camera pixels. The image is 989x650.
Deciding on what section is in best focus (and which image is in best focus) is not easy, and I may want to revisit this someday. I managed to crop out the 1084x722 pixels below from what looked to me like the part of the image with best focus.
Here we end up with a 1562x1042 pixel crop.
Close scrutiny shows diffraction limiting the resolution,
which is what we hope for really.
This image is 0.38 mm top to bottom.
Although the image you see here is bigger than the others, it is still 1:1 pixels from camera to the cropped image with no scaling applied. I just cropped more.
As an amusing calculation. If the field is 2mm and the camera sensor has 5464 pixels, we are putting a 0.366 micron subject distance on a camera pixel.
Prices for these right now (1-2025) at Edmund Optics are:
5x 0.14 NA -- $770 ($191) 10x 0.28 NA -- $1040 ( ?? ) 20x 0.42 NA -- $2440 ($524)The number in parenthesis is the price I paid on Ebay for these. My 10x was bought at least 5 years ago and I have entirely forgotten what I paid, probably around $500. You could argue that the money I saved on the 20x easily pays for another new 10x on Edmund.
Tom's Computer Info / tom@mmto.org